Composite

Part:BBa_K1992006:Design

Designed by: Inbal Adir,Shilo Ohayon   Group: iGEM16_Technion_Israel   (2016-10-14)


Histamin-Tar GFP tagged expression system (promoter+RBS+coding+terminator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1355
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2411
    Illegal SapI.rc site found at 184


Design Notes

none


Source

rhlR sequence and plasmid was recived form Prop. Roee Amit lab

References